Journal: BMC Oral Health

Article Title: Chronic intermittent hypobaric hypoxia attenuates root resorption following delayed tooth replantation by upregulating OPG/RANKL signaling pathway

doi: 10.1186/s12903-026-08135-7

Figure Lengend Snippet: CIHH downregulates RANKL expression and increases the OPG/RANKL ratio in delayed replanted teeth. A Immunohistochemistry staining showing OPG expression in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. Scale bar: 100 μm. B Immunohistochemistry staining showing RANKL expression in the root and periapical tissue of the NN and CIHH groups. Scale bar: 100 μm. C - D Representative Western blot bands ( C ) and quantitative analysis ( D ) showing OPG and RANKL protein expressions in the root and periapical tissue of NN and CIHH groups of delayed replanted teeth. D dentin; P: periodontal ligament; B: bone

Article Snippet: Membranes were blocked with 5% skim milk and incubated with a mouse anti-OPG primary antibody (1:1000, Huabio, China) or a rabbit anti-RANKL primary antibody (1:1000, Huabio, China) overnight at 4 °C.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot